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. 2001 Jan;12(1):171–184. doi: 10.1091/mbc.12.1.171

Figure 10.

Figure 10

Immunoblot of cells transfected with different ratios of tau isoforms. Lysates were prepared from cells 2 days after transfection with different GFP–tau isoforms. Lane 1: Tranfection with EGFP–tau-3R (6 μg DNA/60-mm dish) alone. Lane 2: Transfection with ECFP–tau-4R (6 μg DNA/60-mm dish) alone. Lane 3: Double transfection with both EGFP–tau-3R (3 μg DNA/60-mm dish) and EGFP–tau-4R (3 μg DNA/60-mm dish). Lane 4: Double transfection with both EGFP–tau-3R (6 μg DNA/60-mm dish) and EGFP–tau-4R (3 μg DNA/60-mm dish). Lane 5: Double transfection with both EGFP–tau-3R (3 μg DNA/60-mm dish) and EGFP–tau-4R (6 μg DNA/60-mm dish). Note that the amount of protein expression in transfected cells approximates the ratios of DNA used for transfection. Molecular weights are indicated on the left.