TABLE 1.

Invasion, translocation, and motility phenotypes of the 11168 variants

Phenotype	Time	Result for strain:		Fold difference
		11168-O	11168-GS
Invasion in INT407 cellsa	30 min	2,800 (793)f	10 (0)	280
	2 h	14,000 (5,291)	4,533 (642)	3
Translocation in Caco-2 cellsb	2 h	33,000 (19,798)	25 (7)	1,320
	6 h	68,500 (4,949)	0 (0)	≥68,500
No. of bacteria/cell in Caco-2 cellsc	2 h	1.48	0.36	4.1
	6 h	1.04	0.31	3.6
Motility
Swarm agard	24 h	21.0 mm (2.7)	0 (0)	NAg
Liquid culturee	14 h	+++	−	NA

^aTotal number of bacteria internalized (similar inocula).

^bTotal number of bacteria harvested from the basolateral side of transwell (similar inocula).

^cNumber of bacteria/cell observed inside or between cells, enumerated by random observation of 26 to 50 cells in 2 TEM fields.

^dHalo size on swarm agar plates.

^eEarly-log-phase cultures assayed by light microscopy. +++, very rapid, darting motility; −, apparent Brownian motion only.

^fNumbers in parenthesis indicate standard deviations.

^gNA, not applicable.