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. 2004 Jan;186(2):307–315. doi: 10.1128/JB.186.2.307-315.2004

TABLE 2.

Primer and probe sequences used in Q-RT-PCR assays

Gene Accession no. Forward primer (+) and anti sense primer (−) Probe (5′ FAMa)
gyrB NC003997.3:4584-6506 (+) ACTTGAAGGACTAGAAGCAG CGAAAACGCCCTGGTATGTATA
(−) TCCTTTTCCACTTGTAGATC
atxA NC001496.1:150042-151469 (+) ATTTTTAGCCCTTGCAC CTTTTATCTCTTGGAAATTCTATTACCACA
(−) AAGTTAATGTTTTATTGCTGTC
acpA NC002146.1:68909-70360 (+) ATTATCTTTACCTCAGAATCAG CAATTTCTGAAGCCATTTCTAATCTT
(−) AACGTTAATGATTTCTTCAG
acpB NC002146.1:49418-50866 (+) TTTTTCAATACCTTGGAACT CTTGAAGAATCATTAGGAATCTCATTACA
(−) AATGCCTTTTAGAAACCAC
capB NC002146.1:56089-57483 (+) TTTGATTACATGGTCTTCC ATAATGCATCGCTTGCTTTAGC
(−) CCAAGAGCCTCTGCTAC
16S rRNA NC003997.3:9335-10841 (+) TTCGGGAGCAGAGTG CAGGTGGTGCATGGTTGTC
(−) AACATCTCACGACACGAG
a

FAM, 6-carboxyfluorescein.