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A: Undifferentiated, (B) Differentiated H9+X cells on same slide and (C) 14 days differentiated H9+X hESC with only one XIST territory. D: Two 1q singals (red) and three XIST genes (green) confirm the trisomy X in H9+X line. The MA-H9 Xi lacks XIST RNA but has methyl-H3K27 and ubiquitin (E) monomethyl-H4K20 (F), and a Barr Body (F-insert).
