TABLE 1.
Summary of XIST territories in undifferentiated hESC cultures
| hESC line (Lab and passage #) | Colonies counted | XIST (+) colonies (%) | XIST (±) colonies (%) | XIST (−) colonies (%) |
|---|---|---|---|---|
| ES01—Ware (P63) | 16a | 100 | ||
| ES02—Ware (P37) (manual) | 15a | 100 | ||
| ES02—Ware (P59) (dispase) | 51 | 96 | 2 | 2 |
| ES03—Ware (P87) | 9a | 100 | ||
| ES03—Ware (P88) | 6a | 100 | ||
| BG03—Ware (P47) | 41 | 96 | 2 | 2 |
| HSF6—Ware (P54) | 35 | 100 | ||
| H9—Ware (P31) | 40 | 12 | 13 | 75 |
| H9—Ware (P35) | 72 | 21 | 13 | 64 |
| H9+X—Ware (P44) | 51 | 4 | 96 | |
| H9+X—Ware (P46) | 41 | 46 | 28 | 26 |
| TE03—Technion (P30) | 5a | 40 | 60 | |
| TE04—Technion (P54) | 3a | 33 | 66 | |
| H9—Stein (P48) | 50 | 100 | ||
| H7—Ware (P41) | 30 | 100 |
hESC lines and lab that supplied them, with passage numbers and sample size. The breakdown of the percentage of colonies on the undifferentiated slide is shown under each category. Colonies in the category XIST (+) are considered to show “precocious inactivation,” where >90% of the cells in the colony contain an XIST RNA territory. Although most colonies in the XIST (−) category contained no cells with XIST territories, the few colonies with <5% XIST positive cells were also included. XIST (±) are those colonies with a mixed population of cells, showing between 10% and 80% of the cells in the colony with XIST RNA territories. These slides contained recently passaged hESC colonies, which consisted of usually between 100 and 300 cells. More than one passage (ES03, UW-H9, UW-H9+X) or culture technique (ES02) were assessed.
The entire slide was scanned and all available colonies on the slide were scored.