TABLE 2.
Summary of hESC X-inactivation status broken down by XIST RNA phenotype
| Precocious X-inactivation | |
| H9—Carpenter (P61–63)a | Hoffman et al. (2005) |
| H7—Andrews (P39)a | Enver et al. (2005) |
| ES01—Ware (P63) | |
| ES02—Ware (P37–59) | |
| ES03—Ware (P87–88) | |
| BG03—Ware (P47) | |
| HSF6—Ware (P54) | |
| Mostly non-precocious colonies (but initiation is possible) | |
| H9—Benvenisty (P45–48)a | Dhara and Benvenisty (2004 |
| H9—Ware (P31–32) | |
| H9 + X—Ware (P44–46) | |
| TE03—Technion (P30) | |
| TE04—Technion (P54) | |
| Demonstrated to initiate Xi upon differentiation | |
| H9—Benvenisty (P45–48)a | Dhara and Benvenisty (2004 |
| H9—Ware (passage: low 30 s) | |
| H9 + X—Ware (passage: low 40 s) | |
| Never inactivates an X-chr | |
| H7—Andrews (P110)a | Enver et al. (2005) |
| H7—Carpenter (P48-72)a | Hoffman et al. (2005) |
| H7—Ware (P41) | |
| Loss of XIST expression after precocious inactivation | |
| H9—Stein (P48) | |
| X-trisomy with only a single Xi | |
| H9 + X—Ware (P44–46) | |
hESC lines grouped by phenotype, the lab it was grown in, and the passages assessed. Seven NIH approved hESC lines show precocious X-inactivation. Seven sublines have low levels of precocious inactivation, but having some colonies with Xi suggests they are capable of inactivation (unlike UW-H7 and UM-H9). Three H9 sublines have been demonstrated to initiate X-inactivation upon differentiation. One subline (UM-H9) may have lost XIST expression following precocious inactivation, and one line (H7) never initiates inactivation upon differentiation.
a
Sublines from previous literature; Hoffman et al. (2005), Dhara and Benvenisty (2004), Enver et al. (2005).