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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Exp Cell Res. 2010 Dec 10;317(6):812–822. doi: 10.1016/j.yexcr.2010.12.002

Figure 2. Knockdown of Arp2 changes cell morphology.

Figure 2

A. A representative Western blot showing knockdown of endogenous Arp2 in CEFs by RNA interference. 40 nM of siRNA (control or Arp2-specific) was used to transfect the CEFs. Three other subunits of the Arp2/3 complex were also analyzed and showed significant reduction. B. Quantitative results of Arp2, Arp3, p34 and p21 subunits of the Arp2/3 complex in Arp2-KD cells (n = 3, error bar; s.e.m). C & D. Representative phase contrast images of live CEFs transfected with control siRNA (C) or Arp2-siRNA (D). E–H: higher magnification phase contrast images of fixed cells transfected with control siRNA (E, F) or Arp2-siRNA (G, H).