FIG. 5.

¹³C-NMR spectra of in vitro reactions. The composition of the reaction mixtures is described under Materials and Methods. Reaction A contained the S. enterica PrpE and PrpC enzymes; reaction B contained the S. enterica PrpE, PrpC, and PrpD enzymes; reaction C contained the S. enterica PrpE, PrpC, and reactivated S. oneidensis AcnD enzymes; reaction D contained the S. enterica PrpE and PrpC enzymes and the S. oneidensis PrpF protein; reaction E contained the S. enterica PrpE and PrpC enzymes and the S. oneidensis AcnD enzyme and PrpF protein. Chemical shifts expressed in parts per million are as follows: [2-¹³C]propionate 30.8 ppm; [2-¹³C]MC, 47.5 ppm; and [2-¹³C]MCA, 141.5 ppm. The portion of the spectra removed contained two glycerol peaks as previously described (17).