TABLE 1.
Strains and plasmids used in this studya
| Strain or plasmid | Genotype | Reference or source |
|---|---|---|
| E. coli strains | ||
| BL21(λDE3) | F−ampT hsdSB(rB− mB+) dcm gal λ(DE3) | New England Biolabs |
| DH5α/F′ | F′/endA1 hsdR17(rK− mK+) supE44 thi-1 recA1 gyrA (Nalr) relA1 Δ(lacZYA-argF)U169 deoR [φ80dlacΔ(lacZ)M15] | New England Biolabs |
| JE4570 | BL21(λDE3)/pPRP62 (S. enterica prpC+ in pET 15b bla+) | |
| JE4744 | BL21(λDE3)/pPRP67 (S. enterica prpD+ in pET15b bla+) | |
| S. enterica strains | ||
| TR6583b | metE205 ara-9 | K. Sanderson via J. Roth |
| Derivatives of TR6583c | ||
| JE3056 | prpB121::Tn10d(Tc) | 18 |
| JE3907 | prpC167 zai-6386::Tn10d(Tc)d | 18 |
| JE3909 | prpD169 zai-6386::Tn10d(Tc) | 18 |
| JE3946 | prpB195 zai-6386::Tn10d(Tc) | 18 |
| JE5993 | acnA2::cat+acnB3::kan+ | 17 |
| JE6501 | JE5993/pBAD30 (bla+) | |
| JE6502 | JE5993/pACN9 (S. enterica acnA+ in pBAD30 bla+) | |
| JE6503 | JE5993/pACN10 (S. enterica acnB+ in pBAD30 bla+) | |
| JE6504 | JE5993/pPRP121 (V. cholerae acnD+prpF+ in pBAD30 bla+) | |
| JE6506 | JE5993/pPRP123 (V. cholerae acnD+prpF+ in pBAD30 bla+) | |
| JE7235 | JE5993/pPRP138 (S. oneidensis acnD+ in pBAD30 bla+) | |
| JE7236 | JE5993/pPRP140 (S. oneidensis acnD+prpF+ in pBAD30 bla+) | |
| JE7357 | JE5993/pPRP163 (E. coli ybhJ+ in pBAD30 bla+) | |
| JE7590 | JE5993/pPRP166 (E. coli ybhHIJ+ in pBAD30 bla+) | |
| Plasmids | ||
| pBAD30 | ParaBAD expression vector, bla+ | 16 |
| pACN9 | S. enterica acnA+ in pBAD30 bla+ | 17 |
| pACN10 | S. enterica acnB+ in pBAD30 bla+ | 17 |
| pPRP12-5.4 | S. enterica prpBCD+ in pSU38 kan+ | 18 |
| pPRP21 | S. enterica prpB+ in pBAD30 bla+ | 18 |
| pPRP36 | S. enterica prpD+ in pBAD30 bla+ | 18 |
| pPRP35 | S. enterica prpC+ in pBAD30 bla+ | 18 |
| pPRP62 | S. enterica prpC+ in pET15b bla+ | 20 |
| pPRP67 | S. enterica prpD+ in pET 15b bla+ | 17 |
| pPRP121 | V. cholerae acnD+ in pBAD30 bla+ | |
| pPRP123 | V. cholerae acnD+prpF+ in pBAD30 bla+ | |
| pPRP138 | S. oneidensis acnD+ in pBAD30 bla+ | |
| pPRP140 | S. oneidensis acnD+prpF+ in pBAD30 bla+ | |
| pPRP141 | S. oneidensis prpB+prpC+acnD+prpF+ in pBAD30 bla+ | |
| pPRP149 | S. oneidensis prpB+ in pBAD18Kan kan+ | |
| pPRP150 | S. oneidensis prpC+ in pBAD18Kan kan+ | |
| pPRP151 | S. oneidensis prpB+prpC+ in pBAD 18Kan kan+ | |
| pPRP152 | S. oneidensis acnD+ in pTYB12 bla+ | |
| pPRP153 | S. oneidensis prpF+ in pBAD18Kan kan+ | |
| pPRP154 | E. coli ybhH+ in pBAD18Kan kan+ | |
| pPRP155 | V. cholerae prpF+ in pBAD18Kan kan+ | |
| pPRP156 | S. oneidensis prpF+ in pTYB12 bla+ | |
| pPRP163 | E. coli ybhJ+ in pBAD30 bla+ | |
| pPRP166 | E. coli ybhHIJ+ in pBAD30 bla+ |
a
All S. enterica strains are derivatives of the S. enterica serovar Typhimurium LT2 strain. Unless otherwise stated, strains and plasmids were constructed during the course of this work.
b
Formerly SA2979.
c
See Table 3 for additional strains (and their genotypes) used in this work.
d
Tn10d(Tc) is an abbreviation of Tn10DEL 16DEL17 (37).