TABLE 2.
Construction of plasmids used in this work
| Plasmid name | Vector backbone | Gene(s) cloned | Method of construction |
|---|---|---|---|
| pPRP117 | pDONR201a | V. cholerae acnD | Gateway BP reactionb |
| pPRP121 | pBAD30 | V. cholerae acnD | Gateway LR reaction |
| pPRP123 | pBAD30 | V. cholerae acnD prpF | KpnI, XbaI cloning |
| pPRP136 | pDONR201 | S. oneidensis acnDc | Gateway BP reaction |
| pPRP138 | pBAD30 | S. oneidensis acnD | Gateway LR reaction |
| pPRP137 | pDONR201 | S. oneidensis acnD prpF | Gateway BP reaction |
| pPRP140 | pBAD30 | S. oneidensis acnD prpF | Gateway LR reaction |
| pPRP141 | pBAD30 | S. oneidensis prpB prpC acnD prpF | SacI, XbaI cloning |
| pPRP149 | pBAD18-Kan | S. oneidensis prpB | SacI, XbaI cloning |
| pPRP150 | pBAD18-Kan | S. oneidensis prpC | SacI, XbaI cloning |
| pPRP151 | pBAD18-Kan | S. oneidensis prpB prpC | SacI, XbaI cloning |
| pPRP152 | pTYB12 | S. oneidensis prpF | BsmI, XhoI cloning |
| pPRP153 | pBAD18-Kan | S. oneidensis prpF | EcoRI, XbaI cloning |
| pPRP154 | pBAD18-Kan | E. colid ybhH | SacI, XbaI cloning |
| pPRP155 | pBAD18-Kan | V. cholerae prpF | KpnI, XbaI cloning |
| pPRP156 | pTYB12 | S. oneidensis acnD | BsmI, XhoI cloning |
| pPRP163 | pBAD30 | E. coli ybhJ | SacI, XbaI cloning |
| pPRP166 | pBAD30 | E. coli ybhH ybhl ybhJ | SacI, XbaI cloning |
a
pDONR201 was purchased from Invitrogen, Carlsbad, Calif.
b
Gateway BP and LR reactions are methods developed by Life Technologies, Invitrogen Corporation.
c
All S. oneidensis organisms are strain MR-1.
d
All E. coli organisms are strain MG1655.