TABLE 3.
Heterologous complementation of S. enterica prp mutant strains
| Strain | Genotypea | Arabinose concentrationb (μM) | Doubling timec (h ± SD) |
|---|---|---|---|
| JE4175 | TR6583 (wild type)/pBAD30 | 500 | 7.3 ± 0.3 |
| JE6107 | JE3909/pPRP36 (S. enterica prpD+) | 500 | 7.6 ± 0.3 |
| JE7280 | JE3909/pPRP138 (S. oneidensis acnD+), pPRP153 (S. oneidensis prpF+) | 500 | 7.9 ± 0.3 |
| JE7232 | JE3909/pPRP140 (S. oneidensis acnD+prpF+) | 500 | 8.0 ± 0.5 |
| JE7227 | JE3909/pPRP141 (S. oneidensis prpB+prpC+acnD+prpF+) | 500 | 9.2 ± 1.0 |
| JE7282 | JE3909/pPRP138 (S. oneidensis acnD+), pPRP155 (V. cholerae prpF+) | 500 | 20.4 ± 1.2 |
| JE7283 | JE3909/pPRP121 (V. cholerae acnD+), pPRP153 (S. cholerae prpF+) | 100 | 11.0 ± 0.5 |
| JE7285 | JE3909/pPRP121 (V. cholerae acnD+), pPRP155 (V. cholerae prpF+) | 500 | 25.8 ± 2.0 |
| JE7234 | JE3909/pPRP123 (V. cholerae acnD+prpF+) | 100 | 13.0 ± 0.4 |
| JE6105 | JE3909/pBAD30 | 500 | NG |
| JE7238 | JE3056/pPRP12-5.4 (S. enterica prpB+prpC+prpD+) | 0 | 15.8 ± 0.5 |
| JE7274 | JE3056/pPRP151 (S. oneidensis prpB+prpC+), pPRP36 (S. oneidensis prpD+) | 100 | 12.7 ± 0.3 |
| JE7275 | JE3056/pPRP151 (S. oneidensis prpB+prpC+), pPRP140 (S. oneidensis acnD+prpF+) | 100 | 19.2 ± 0.4 |
| JE7277 | JE3056/pPRP151 (S. oneidensis prpB+prpC+), pPRP123 (V. cholerae acnD+prpF+) | 100 | 14.7 ± 0.5 |
| JE7239 | JE3056/pPRP141 (S. oneidensis prpB+prpC+acnD+prpF+) | 100 | 27.0 ± 1.4 |
| JE5585 | JE3056/pBAD30 | 500 | NG |
| JE7242 | JE3056/pPRP151 (S. oneidensis prpB+prpC+) | 500 | NG |
| JE5297 | JE3946/pPRP21 (S. oneidensis prpB+) | 500 | 9.7 ± 0.3 |
| JE7252 | JE3946/pPRP149 (S. oneidensis prpB+) | 100 | 11.3 ± 0.4 |
| JE7253 | JE3946/pPRP151 (S. oneidensis prpB+prpC+) | 100 | 10.5 ± 0.5 |
| JE5296 | JE3946/pBAD30 | 500 | NG |
| JE7270 | JE3907/pPRP35 (S. enterica prpC+) | 100 | 11.0 ± 0.2 |
| JE7254 | JE3907/pPRP150 (S. oneidensis prpC+) | 500 | 12.6 ± 0.3 |
| JE7255 | JE3907/pPRP151 (S. oneidensis prpB+prpC+) | 500 | 11.6 ± 0.4 |
| JE7251 | JE3907/pBAD30 | 500 | NG |
All plasmids except pPRP12-5.4 (S. enterica prpBCD+) contain arabinose-inducible promoters. Where two plasmids are indicated, they are of different incompatiblity groups. The following strains were used to construct the strains tested: JE3909 [metE205 ara-9 prpD169 zai-6386::Tn10d(Tc)], JE3907 [metE205 ara-9 prpC167 zai-6386::Tn10d(Tc)], JE3056 [metE205 ara-9 prpB121::Tn10d(Tc)], and JE3946 [metE205 ara-9 prpB195 zai-6386::Tn10d(Tc)].
Each construct was tested in media with 100 and 500 μM arabinose; the highest growth rate was included in this table.
JE7230 (JE3909/pPRP138 [S. oneidensis acnD+]), JE7271 (JE3909/pPRP153 [S. oneidensis prpF+]), JE7233 (JE3909/pPRP121 [V. cholerae acnD+]), JE7273 (JE3909/pPRP155 [V. cholerae prpF+]), JE7523 (JE3909/pPRP166 [E. coli ybhHIJ+]), JE7356 (JE3909/pPRP163 [E. coli. ybhJ+]), JE7272 (JE3909/pPRP154 [E. coli ybhH+]), or JE7359 (JE3909/pPRP154 [E. coli ybhH+], pPRP163 [E. coli ybhJ+]) did not grow in a propionate medium supplemented with 500 μM arabinose. NG, no growth observed (doubling times greater than 50 h).