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Specific activities of AcnD and AcnAa with different substrates
| Substrate | AcnD | AcnA |
|---|---|---|
| Citrate | 2.9 ± 0.5 | 26.5 ± 4.0 |
| cis-Aconitate | 5.0 ± 0.2 | 68.5 ± 7.4 |
| Isocitrate | NDb | 43.4 ± 3.1 |
| 2-MC | 7.8 ± 0.4 | ND |
| 2-MIC | ND | 12.7 ± 1.4 |
AcnD and AcnA were reconstituted as stated in Materials and Methods.
ND, no detectible activity observed (limits of detection were 0.5 μM/min for cis-aconitate conversion and 0.4 μM/min for 2-MCA formation) under the conditions tested.
