TABLE 1.
Purification of natural and recombinant SUH
| Enzyme | Stepa | Protein (mg) | Total activity (U) | Sp act (U/mg) | Purification (fold) | Recovery (%) |
|---|---|---|---|---|---|---|
| SUH | CPX | 2,270 | 180 | 0.079 | 1 | 100 |
| ASF | 840 | 158 | 0.19 | 2.4 | 88 | |
| AEC | 320 | 123 | 0.38 | 4.8 | 68 | |
| HAC | 99 | 91.7 | 0.93 | 12 | 51 | |
| GPC | 9.6 | 40.9 | 43 | 54 | 23 | |
| HIC | 0.76 | 20.2 | 27 | 342 | 11 | |
| FPLC | 0.097 | 6.1 | 64 | 810 | 3.4 | |
| rSUH | CPX | 68 | 387 | 5.7 | 1 | 100 |
| Ni-NTA | 31 | 372 | 12 | 2.1 | 96 | |
| HIC | 8.8 | 253 | 29 | 5.1 | 65 | |
| FPLC | 2.2 | 148 | 67 | 12 | 38 |
a
CPX, crude protein extract; ASF, ammonium sulfate fractionation; AEC, anion-exchange chromatography; HAC, hydroxyapatite chromatography; GPC, gel permeation chromatography; HIC, hydrophobic interaction chromatography; Ni-NTA, Ni-nitrilotriacetic acid affinity chromatography.