Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Mar 15;22(6):736–747. doi: 10.1091/mbc.E10-08-0724

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Matsushita et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,“ “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 3: — Interaction of Gipie with GRP78. (A) Interaction of endogenous Gipie and GRP78. Lysates from U937 cells (left panels) and HUVECs (right panels) were immunoprecipitated with anti-Gipie, anti-GRP78, and normal rabbit IgG antibodies, followed by Western blot analysis using the indicated antibodies. (B) Colocalization of Gipie with GRP78 in HUVECs. HUVECs were fixed and stained with anti-Gipie (green) and anti-KDEL (red) antibodies, showing the localization of Gipie in GRP78-positive ER structures (white box). Scale bars: 10 μm (top panel), 1 μm (bottom panel). (C) Fragments of Gipie tagged with the V5 epitope used in this study. (D) The C-terminal domain (CT) of Gipie is required for interaction with GRP78. Lysates from COS7 cells transfected with fragments of Gipie were immunoprecipitated with anti-V5 antibody, followed by Western blot analysis with anti-KDEL antibody (bottom panels). Expression of Gipie and GRP78 fragments was monitored by Western blot analysis using the indicated antibodies (top and middle panels, respectively). TCL, total cell lysates. CT-V5, C-terminal fragment of Gipie. NT-CC-V5, the mutant lacking the C-terminal fragment of Gipie.