. 2001 Jan;12(1):185–199. doi: 10.1091/mbc.12.1.185

Table 2.

Methylation is required for A/C heterodimer binding to Bα subunit but not for its association with MT, striatin, or SG2NA

C subunit^a	Methylation competence^b	Bα subunit binding^b ^c	Striatin binding^d	SG2NA binding^d	MT binding^c ^f
wt	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺	⁺⁺⁺	wt
301stop	−	−	⁺⁺⁺^e	⁺⁺⁺^e	wt
L309Δ	−	−	⁺⁺⁺⁺	⁺⁺⁺⁺	wt
T301D	⁺⁺⁺	⁺	n.a.	n.a.	wt
T304D	⁺⁺⁺	−	⁺⁺⁺	⁺⁺⁺	wt
T304A	⁺⁺⁺	⁺⁺⁺⁺	⁺⁺⁺	⁺⁺	wt
T304N	⁺⁺⁺	−	n.a.	n.a.	wt
T304K	⁺⁺⁺	−	n.a.	n.a.	wt
Y307E	−	−	⁺⁺⁺⁺	⁺⁺⁺⁺	wt
Y307F	⁺⁺	⁺⁺⁺⁺	⁺⁺⁺⁺	⁺⁺⁺⁺	wt
Y307Q	−	−	n.a.	n.a.	wt
Y307K	−	−	n.a.	n.a.	wt
H59Q	−	−	⁺⁺	⁺	wt
H118Q	−	−	n.a.	n.a.	wt
D85N	−	−	n.a.	n.a.	wt
R89A	−	−	n.a.	n.a.	wt

The description of the C subunit mutants and their nomenclature, as well as the abbreviations for the amino acid residues are the same as in the legend to Table 1 except that Δ indicates deletion of the specified residue. 301stop indicates the replacement of residue 301 with a stop codon.

For these assays: ++++, > wt level; +++, 71–100% of the wt level; ++, 41–70% of the wt level; +, 15–40% of the wt level; −, <15% of the wt level; n.a., not assayed.

Bα subunit and MT-binding results for mutants other than L309Δ were taken from Ogris et al. (1999a, 1999b, 1997) and were estimated from autoradiograms.

For these assays, the symbols are equivalent to footnote b except that ++++ indicates ≥twofold the wt level because lesser increases may not be significant.

These values were estimated from autoradiograms.

Because of the variability of MT expression in each of the MT transfected cell lines, only qualitative data were obtained. wt indicates that MT associated with each of these mutants at near-wt levels.