TABLE 3.

Detection of gene expression in R. prowazekii by real-time PCR

Gene	Madrid E		Breinl		Expression (Breinl vs. Madrid E)c
	CTa	x ± SDb	CT	x ± SD
virB4	36.65	36.41 ± 0.21	34.41	34.74 ± 0.40	2.7
	36.26		34.62
	36.32		35.19
ftsk	36.30	36.19 ± 0.10	35.75	35.47 ± 0.25	1.4
	36.15		35.40
	36.11		35.27
rfbE	40.88	42.00 ± 4.04	36.98	40.22 ± 4.08	2.9
	38.64		38.87
	46.49		44.80
rpoH	33.29	33.59 ± 0.39	32.71	32.84 ± 0.22	1.4
	33.45		32.72
	34.03		33.10
lpxA	35.03	35.03 ± 0.23	32.30	32.69 ± 0.41	4.2
	35.26		32.67
	34.81		33.11
rp105	33.76	33.65 ± 0.11	32.96	32.97 ± 0.08	1.3
	33.54		33.06
	33.64		32.90
ampG2	35.11	35.05 ± 0.17	31.69	31.86 ± 0.24	7.6
	35.18		31.76
	34.85		32.14
pal	30.04	30.09 ± 0.35	27.55	27.77 ± 0.26	4.2
	29.77		27.71
	30.46		28.05
iles	34.60	34.44 ± 0.30	32.79	33.15 ± 0.34	2.0
	34.09		33.20
	34.63		33.47
uvrB	39.04	39.39 ± 0.31	36.00	35.83 ± 0.15	9.9
	39.61		35.71
	39.52		35.78
16S rRNAd	18.42	18.38 ± 0.07	18.00	18.12 ± 0.14
	18.30		18.08
	18.43		18.28

^aC_T represents the fractional cycle number at which a significant increase in fluorescence signal above a threshold signal (horizontal zero line) can first be detected.

^bx, average value.

^cUsing the comparative (ΔΔC_T) method.

^dInternal control.