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. 2011 Mar 15;22(6):842–857. doi: 10.1091/mbc.E10-07-0570

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© 2011 Rossi and Brennwald. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — The myo2-iq1,5 mutant shows a pronounced cold-sensitive secretory defect in post-Golgi transport. (A) Wild-type and the myo2-iq1,5 mutant were grown at the permissive temperature of 32°C and then shifted to the restrictive temperature of 25°C. Growth at both temperatures was monitored at 3-h intervals in YPD media. (B) Graphs show results of invertase and Bgl2 assays conducted on wild-type, myo2-iq1,5, and the sec4-P48 mutants after a shift to the restrictive temperature of 25°C for 8 h. Error bars represent the SD from three independent experiments. A representative blot of a Bgl2 assay is shown. (C) myo2-iq1,5 cells grown under permissive conditions (32°C) or grown at 32°C and then shifted to restrictive conditions (25°C) for 8 h were fixed and processed for electron microscopy.