FIGURE 5:

Sro7 forms large clusters of post-Golgi vesicles when expressed in the myo2iq-1,5 mutant under a moderate promoter. (A) Wild-type, myo2-iq1,5, and sec15-1 mutant cells were transformed with a plasmid expressing Sro7 behind a tetracycline-repressible promoter (CEN) or vector only. Four independent transformants were picked into microtiter wells and transferred to YPD in the presence or absence of doxycycline. (B) Wild-type and myo2-iq1,5 mutant cells containing a Sro7 behind a tetracycline-repressible promoter (CEN) were transformed with GFP-SEC4 (CEN). Cells were grown in selective media in the presence of doxycycline and then shifted to media lacking doxycycline to induce Sro7 expression for 8 h at 25°C. Cells were then fixed and processed for fluorescence microscopy (bar, 1 μm) and (C) electron microscopy.