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. 2011 Mar 15;22(6):842–857. doi: 10.1091/mbc.E10-07-0570

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© 2011 Rossi and Brennwald. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Sec15 and Sro7 drive formation of clusters of post-Golgi vesicles in wild-type cells when expressed from a strong promoter. (A) Vesicle clustering is Rab dependent and Sec9 t-SNARE independent. Wild-type, sec4-8, sec2-41, and sec9-4 mutant strains were transformed with GAL-SRO7 (Yip) or GAL-SEC15 (Yip). Four individual colonies were picked into microtiter wells and then transferred onto YP plates containing dextrose or galactose. (B) Glass bead lysis and Western blot analyses was conducted to compare expression levels of Sro7 using anti-Sro7 and anti-Sso (control) rabbit polyclonal antibodies. (C) Wild-type strains expressing Sec15 or Sro7 from the galactose-inducible promoter (YIP) were grown in raffinose and then induced in galactose for 10 h at 25°C before fixing and processing for electron microscopy. Bar 1 μm. A high magnification is shown as an inset on the extreme right side of the figure.