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. 2011 Mar 15;22(6):842–857. doi: 10.1091/mbc.E10-07-0570

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© 2011 Rossi and Brennwald. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Overexpression of Sec15 or Sro7 correlates with the appearance of a bright patch of Sec4 in the cell. Wild-type, sec9-4, and sec4-8 strains transformed with (A) GAL-SEC15 (YIP) or (B) GAL-SRO7 (YIP) were grown in raffinose and then induced with galactose for 10 h at 25°C. Cells were then fixed for immunofluorescence microscopy and analyzed with monoclonal anti–mouse Sec4 antibodies and polyclonal anti–rabbit Sec15 or Sro7 antibodies. Bar 1 μm.