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. 2011 Mar 15;22(6):858–866. doi: 10.1091/mbc.E10-09-0768

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© 2011 Pollock et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — The MRP RNA–CUGBP–PTB complex is detected in fraction 11. (A) PTB and CUGBP proteins are detected in fractions of a 5–45% glycerol gradient with anti-PTB and anti-CUGBP antibodies, and MRP RNA is detected with Northern blot. (B) Fractions are blotted with anti-Rpp30 and anti-hTERT antibodies. (C) PTB is precipitated from fractions 6/7 and 11, and MRP is detected with RT-PCR. (D) PTB is precipitated from fraction 11, and anti-CUGBP is used to blot. Ten percent of material used in IP was loaded as input.