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. 2011 Mar 15;22(6):858–866. doi: 10.1091/mbc.E10-09-0768

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© 2011 Pollock et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Immobilized PNC proteins are able to recruit additional PNC components. Immunofluorescence microscopy of HeLa cells expressing GFP-LacI protein and/or Cherry-LacI is represented. (A, top) HeLa cells are transfected with Cherry-LacI (red) and endogenous PTB is stained (green). (A, bottom, marked with arrows) Colocalization of transfected GFP-LacI-CUGBP (green) and Cherry-LacI (red) is visualized. (B) HeLa cells are cotransfected with Cherry-LacI (red) and either GFP-LacI-PTB or GFP-LacI-CUGBP (green) and immunostained for endogenous CUGBP or PTB (blue), respectively. (C) HeLa cells were transfected with GFP-LacI-CUGCP (green) and endogenous MRP RNA was in situ labeled (red) to visualize colocalization. Scale bar = 10 μm. Arrows point to colocalization of transfected proteins with endogenous PNC components.