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. 2011 Mar 15;22(6):858–866. doi: 10.1091/mbc.E10-09-0768

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© 2011 Pollock et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — The dynamics of GPF-CUGBP and GFP-PTB differ between the PNC and nucleoplasm. (A) Select images are shown from a representative FRAP time series acquisition of transiently transfected GFP-PTB and (B) GFP-CUGBP in HeLa cells (n = 18). Circles in the prebleached image demarcate the two bleached regions subject to quantification in each cell. Scale bar = 10 μm. (C) FRAP studies on cells that were transfected with GFP-PTB or (D) GFP-CUGBP were averaged, and percentage recovery was plotted over time. (E) Box-and-whisker plots depict the distribution of the half-recovery times for GFP-PTB and GFP-CUGBP FRAP studies. At the PNC, GFP-PTB (t_1/2 = 24.9) and GFP-CUGBP (t_1/2 = 48.3) recovered more slowly than nucleoplasmic GFP-PTB (N Near t_1/2 = 2.6, and N Far t_1/2 = 5.3) and GFP-CUGBP (N Near t_1/2 = 20.0, and N Far t_1/2 = 19.0). Three ROI were quantified for each study: the PNC, nucleoplasm near the PNC (N Near), and nucleoplasm further from the PNC (N Far). Each study was corrected for photofading and normalized to the prebleached image before averaging. *p < 0.001; Student’s t test.