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A working model of the PNC. (A) Fluorescence microscopy was used to show localization of the PNC, labeled with PTB (red) and the MRP DNA locus (green). Scale bar = 10 μm. (B) High-resolution microscopy was used to visualize the PNC of cells triple labeled with MRP (red), BrU (green), and PTB (blue). Scale bar = 1 μm. (C) We propose that the novel RNPs associated with the PNC may be involved in processing the newly synthesized RNA at a discreet DNA locus/loci.
