Table 2.
Yields (from 50 g beans) and hemagglutinating activities of various chromatographic fractions obtained during different steps of purification of mini-black soybean hemagglutinin. HA: hemagglutinating.
| Fraction | Total protein (mg) | Total HA activity (u) | Specific HA activity (u/mg) | Recovery of HA activity(%) | Fold of purification |
|---|---|---|---|---|---|
| Extract | 9800 | 87220 | 8.9 | 100 | 1 |
| Q | 625 | 30010 | 48 | 34.4 | 5.3 |
| SP3 | 23 | 5888 | 256 | 6.8 | 28.8 |
| S | 18 | 5526 | 307 | 6.3 | 34.5 |