TABLE 1.
Strains, plasmids, and oligonuclotides used in this work
| Strain, plasmid, or oligonucleotide | Characteristic(s) or sequence | Source or reference |
|---|---|---|
| Strains | ||
| MG1655 | λ−rph-1 | Laboratory stock |
| NK6024 | λ− Δ(gpt-lac)5 pheA18::Tn10 relA1 spoT1 thi-1 | B. J. Bachmann |
| TK1135 | MG1655 ΔaroP mtr24 ΔpheP Δtna ΔtyrP | 23 |
| TK1170 | MG1655 ΔaroP Δmtr ΔpheP Δtna ΔtyrP | This study |
| TK1173 | TK1170 pheA18::Tn10 | This study |
| YG74 | MG1655 ΔaroP ΔlivHMGF ΔpheP | This study |
| YG106 | MG1655 ΔaroP ΔbrnQ ΔpheP | This study |
| YG108 | MG1655 ΔaroP ΔbrnQ ΔlivHMGF | This study |
| YG109 | MG1655 ΔbrnQ ΔlivHMGF ΔpheP | This study |
| YG195 | MG1655 ΔaroP ΔbrnQ Δmtr ΔpheP Δtna ΔtyrP | This study |
| YG198 | MG1655 ΔaroP ΔbrnQ ΔlivHMGF Δmtr ΔpheP Δtna ΔtyrP | This study |
| YG201 | MG1655 ΔaroP ΔbrnQ ΔlivHMGF ΔpheP | This study |
| YG208 | MG1655 pheA18::Tn10 | This study |
| YG210 | YG106 pheA18::Tn10 | This study |
| YG211 | YG108 pheA18::Tn10 | This study |
| YG212 | YG109 pheA18::Tn10 | This study |
| YG213 | YG201 pheA18::Tn10 | This study |
| YG228 | MG1655 ΔaroP ΔbrnQ Δ(livJ-yhhK-livKHMGF) ΔpheP | This study |
| YG256 | YG228 ΔtyrP | This study |
| Plasmids | ||
| pACYC177 | p15A replicon bla+kan+ | 6 |
| pCP20 | pSC101 replicon (Ts) bla+cat+ Flp(λRp) cI857 | 7 |
| pKD13 | oriRγ bla+ FRT-kan+-FRT | 12 |
| pKD46 | oriR101 repA101(Ts) bla+araC+gam+-bet+-exo+ (araBp) | 12 |
| pMBO131 | Mini-F replicon cat+ | 30 |
| pMW118 | pSC101 replicon bla+lacZα+ | Nippon Gene |
| pYG218 | pSC101 replicon bla+livH+M+G+F+ | This study |
| pYG237 | Mini-F replicon kan+livJ+ | This study |
| pYG239 | Mini-F replicon kan+livK+ | This study |
| pYG249 | Mini-F replicon kan+; the 1.7-kb NheI-BamHI fragment containing the kan+ gene was recovered from pACYC177, blunt ended, and then ligated with the 4.8-kb ScaI-XhoI (blunt-ended) fragment of pMBO131 | This study |
| Oligonucleotides | ||
| brnQ-F | 5′-GATTAGCCATGTCTTTTTCACGGAA-3′ (for cloning the brnQ gene; upstream end) | |
| brnQ-R | 5′-ATGCTTTGATCCCGTCGAGAATAC-3′ (for cloning the brnQ gene; downstream end) | |
| livH-F | 5′-AGGTTACCTTATGTCTGAGCAG-3′ (for cloning the livHMGF genes; upstream end) | |
| livF-R | 5′-CGGTTTCATCGTTTATCTCTCTT-3′ (for cloning the livHMGF genes; downstream end) | |
| liv-F1 | 5′-CCAATCCCCACGCAGATTGTTAATAAACTG-3′ (for cloning the livJ-yhhK-livK genes and the livJ-yhhK-livKHMGF genes; upstream end) | |
| liv-R1 | 5′-GTGAGGGAAAATGGGAGATGGGGC-3′ (for cloning the livJ-yhhK-livKHMGF genes; downstream end) | |
| liv-R2 | 5′-CAATCATATAAACCTCGCCGTGGG-3′ (for cloning the livJ-yhhK-livK genes; downstream end) | |
| mtr-1 | 5′-CACCGTCGCTGCTTGGCGGCGTGGTGATTATCGGCGGCACGTGTAGGCTGGAGCTGCTTC-3′ (for disruption of the mtr gene; upstream end) | |
| mtr-2 | 5′-GCTGCCAAAGCGTTTACGCGATGCACGGGCTAACAGCGCCATTCCGGGGATCCGTCGACC-3′ (for disruption of the mtr gene; downstream end) | |
| pKD13-1 | 5′-GTGTAGGCTGGAGCTGCTGCTTC-3′ (for amplifying the kanamycin resistance gene; upstream end) | |
| pKD13-4 | 5′-ATTCCGGGGATCCGTCGACC-3′ (for amplifying the kanamycin resistance gene; downstream end) |