FIGURE 9.

Effect of compounds 16 and 17 on cell viability. Percentage of apoptotic (A) and survival cells (B) after incubation with compounds 16 and 17 is shown. Early apoptotic cells were quantified as the percentage of total population of annexin V⁺,PI⁻ cells, late apoptotic, and/or necrotic cells as annexin V⁺ and PI⁺, and viable nonapoptotic cells as annexin V⁻ and PI⁻ at 24 h of culture of human neutrophils. The columns are the means ± S.E. of 7–9 independent replicates. *, p < 0.05 compared with vehicle. Representative flow cytometry panels showing the effects of compounds 16 and 17 on human neutrophil apoptosis have been included.