FIGURE 4.
LCE-BoTIMA potently blocks neuromuscular transmission, an effect not reversed by 4-aminopyridine, unlike that of BoNTA. A, mouse hemi-diaphragms were incubated with each toxin, and the paralysis times were recorded. The data were fitted with power functions (solid lines) as described (16); BoNTE at 1 nm deviated from this relationship because of approaching saturation. BoTIMA (10 nm) did not cause any paralysis within 3 h. B, mouse diaphragms were incubated with 1 nm BoNTA or LCE-BoTIMA. After reaching ∼90% blockade of initial muscle tension, 10 mm 4-aminopyridine (4-AP) was added to the bath. All of the values are the means ± S.E. (n ≥ 3). In some cases, the error bars are encompassed by the symbols. nBoNT and BoNT represent natural and recombinant toxin, respectively.