TABLE 1.
Mouse lethalities of purified toxins and variants
The doses are those used in the DAS assay.
| Toxin | Lethality | TDmaxa |
|---|---|---|
| mLD50 units/mgc | pg [mLD50 units]c | |
| Natural (n)BoNTAb | 3 × 108 | 20 [6] |
| Natural (n)BoNTEb | 1 × 108 | 70 [7] |
| BoNTA (WT) | 2 × 108 | 10 [2] |
| BoNTA (ΔN6) | 4 × 106 | 500 [2] |
| BoNTA (AA) | 3 × 104 | 1 × 105 [3] |
| BoNTEAb | 7 × 106 | 1140 [8] |
| N8-BoNTEA | 4 × 105 | 5000 [2] |
| BoTIMA | <3 × 103 | NA |
| LCE-BoTIMA | 7 × 107 | 8 [0.5] |
| LCE-BoTIMA (AA) | 3 × 104 | 1 × 105 [3] |
a TDmax is the maximal tolerated dose that could be injected intramuscularly without producing systemic symptoms (e.g. complete immobilization of injected leg, lower abdomen contraction, and subsequent general paralysis).
b Data from Ref. 22.
c 1 mLD50 unit is the lowest dose of toxin that killed 50% of a group of four mice within 4 days after intraperitoneal injection.