FIGURE 2.

Cloning and expression of canine galectins. A, RNA was prepared from MDCK cells (M) and a commercial source of dog jejunum (J) and amplified by RT-PCR. Amplified DNA (∼200 bp) was visible by ethidium bromide staining for Gal-1, -3, -8, and -9 after 28 cycles, while amplified cDNAs for Gal-2, -4, -7, and -12 required nested primers. See supplemental Table S1 for primer sequences. B, cDNAs for Gal-1, -2, -3, -4, -7, -8, -9, and -12 were expressed in bacteria as GST fusion proteins and subjected to SDS-PAGE and Coomassie staining. The N-terminal and C-terminal carbohydrate recognition domains of Gal-9 were individually expressed as GST-Gal-9N and GST-Gal-9C, respectively.