Figure 11.
sda1-2 cells released from a G0 arrest at the restrictive temperature fail to induce increased synthesis of rRNA and proteins. (A) Total RNA was isolated from wild-type or sda1-2 cells at the indicated times after release from a G0 arrest at the restrictive temperature. The RNA was then separated by electrophoresis, transferred to a nylon membrane, and stained with methylene blue. (B) The rate and pattern of protein synthesis were measured at each of the indicated time points after release from a G0 arrest by giving cells a 15-min pulse of 35S-labeled amino acids. The synthesized proteins were resolved by gel electrophoresis and were visualized by autoradiography. In addition, total TCA-precipitable counts were measured, and total proteins were visualized by Coomassie blue staining of a polyacrylamide gel.