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Sda1 is absent in G0 cells and appears before Start. (A) Rapidly growing wild-type cells were arrested in G0, G1, or mitosis and probed for the presence of Sda1, Nap1, and Clb2 by Western blotting. (B) G0-arrested wild-type cells containing a HA-tagged version of CLN2 were released into fresh media with or without 1 μg/ml α factor at 30°C and probed for the appearance of Sda1 and Cln2 by Western blotting.
