Figure 7.

Behavior of the Sda1 protein and mRNA at the restrictive temperature. (A) G0-arrested wild-type and sda1-2 cells were released into fresh media at the permissive (20°C) or restrictive temperature (37°C) and assayed for the appearance of Sda1 protein by Western blotting. (B) Rapidly growing wild-type and sda1-2 cells were shifted from the permissive temperature to the restrictive temperature and samples were taken every 5 h to assay for the presence of the Sda1 protein by Western blotting. At each time point, cells were diluted to O.D.₆₀₀ of 0.6 to normalize total protein in each sample. (C) G0-arrested wild-type and sda1-2 cells were released into fresh media at the restrictive temperature (37°C) and monitored for the appearance of the SDA1 transcript by Northern blotting.