TABLE 1.
Random mutants of C-terminal SK (residues 210–414)
The first column represents selected random mutants (RM), and the second column represents the corresponding residues identified in the random mutants after DNA sequencing. The cofactor activities of the random mutants in complex with HPN (SK/SK*·HPN) were determined for substrate HPG (see “Experimental Procedures”) and were calculated as percent change in activity compared to wtSK, taken as 100%.
| Random mutant | Mutation | Specific activity (Pathway II) |
|---|---|---|
| % | ||
| wt SK | wt | 100 |
| RM 1 | P329L | 0.5 |
| R324G | ||
| RM 2 | G344D | 14 |
| RM 3 | N265Y | 3 |
| R330C | ||
| E355D | ||
| RM 4 | H226L | 10 |
| D328N | ||
| H381Q | ||
| RM 5 | D322R | 5 |
| D328H | ||
| RM 6 | K334E | 12 |
| RM 7 | R324G | 8 |
| RM 8 | D328G | 10 |
| RM 9 | F287S | 25 |
| N339S | ||
| D347V | ||
| RM 10 | D322G | 7.5 |
| R330H | ||
| R363L | ||
| T403R |