FIGURE 7.

Effect of MeHg on TrxR1. A, TrxR1 activity 8 h after exposure to MeHg. Pretreatment with 30 nm sodium selenite could not suppress the decrease in TrxR1 activity after 0.4 μm MeHg exposure. However, pretreatment with 60 nm sodium selenite rescued the MeHg-induced decrease in TrxR1 activity. Values shown are means ± S.E. of triplicate samples in 3 separate experiments. B, Western blots of C2C12-DMPK160 cells were analyzed with the indicated antibody probes. The densitometric quantification of TrxR1 protein normalized to the α-tubulin protein is represented as a fold-increase over the control. Representative images of three samples were shown with quantitative data (means ± S.E.).