FIGURE 2.
ITC titration of 5-μl aliquots of a 0.29 mm NADP+ solution into a solution of 0.051 ± 0.005 mm of wild-type hAR (pH 6.5) and 25 °C, shown in top panel. The binding isotherm for the titration (■) along with a least squares fit of a one to one binding model to the binding isotherm (—). μW, microwatts.