TABLE 1.
Comparison of catalytic activity of wild-type and C298S mutant hAR for carbonyl reduction and alcohol oxidation reactions at 25 °C
The uncertainties are S.D. of the average value.
| hAR Protein | Substrate | Km | kcat | kcat/Km |
|---|---|---|---|---|
| s−1 | ||||
| Wild-type | dl-Glyceraldehyde | 0.1 ± 0.01 mm | 1.55 ± 0.01 | 14.5 mm−1s−1 |
| C298S | 1.2 ± 0.05 mm | 3.72 ± 0.3 | 3.10 mm−1s−1 | |
| Wild-type | d-Glucose | 55.0 ± 6.0 mm | 0.64 ± 0.05 | 0.011 mm−1s−1 |
| C298S | 800 ± 50.0 mm | 0.42 ± 0.04 | 0.0005 mm−1s−1 | |
| Wild-type | HNE | 48.0 ± 4.0 μm | 0.90 ± 0.06 | 0.019 μm−1 s−1 |
| C298S | 110.0 ± 8.0 μm | 0.60 ± 0.02 | 0.006 μm−1 s−1 | |
| Wild-type | Benzyl alcohol | 1.62 ± 0.1 mm | 0.35 ± .025 | 0.215 mm−1s−1 |
| C298S | 4.55 ± 0.3 mm | 0.42 ± .02 | 0.092 mm−1s−1 |