FIGURE 3.

Classical CaMKII inhibitors enhance Ca²⁺-independent binding of CaM to AKAP79. AKAP79(31–52) was incubated with CaM (1 μm; Input lane, 50 ng) and KN-62 at the indicated concentrations in the absence or presence of Ca²⁺. A, top, representative blots of CaM binding to AKAP79(31–52) in 1 mm EGTA (upper) or 100 μm Ca²⁺ (lower). Bottom, summary data normalized to respective controls (n = 3–6; for EGTA: 10 nm–10 μm KN-62, p < 0.05). B, same as A but with KN-93 (n = 5–7; for EGTA: 1 nm, 100 nm, and 1 μm, p < 0.01; 10 nm, p < 0.05). C and D, same as above except with KN-92 (n = 6) and CaMKIINtide (n = 5–6), respectively. All graphs depict mean ± S.E.