TABLE 1.
E. coli strains and plasmids used in this study
| Strain/Plasmid | Genotype | Reference |
|---|---|---|
| Strain | ||
| AB2847 | aroB thi tsx malT | (1) |
| BL21(DE3) | F−ompT gal dcm lon hsdB (rB− mB−) λ(DE3) lacI lacUV5-T7 gene 1 | (50) |
| BL21 fhuA | BL21(DE3) fhuA | (11) |
| MB97 | AB2847 ΔfhuA | (54) |
| K458 | AB2847 fkpA43 (tolM-ts) | (20) |
| Mo3 | AB2847 fkpA40 | (10) |
| ES965 | KTu50 pES7 cba cbi on pACYC184 | (51) |
| DH5α | supE44 ΔlacU169 [Φ80lacZΔM15] hsdR17 recA1 endA1 gyrA thi-1 relA1 | (52) |
| SIP1275 | AB2847 fkpA313 | (11) |
| Plasmid | ||
| pMLD237 | cma encoding Cma-Hisa | (3) |
| pSP127/56 | pET25b encoding FkpA313-Hisb | (11) |
| pYH15 | pET25b encoding FkpA-Hisb | (11) |
| pYH16 | pET25b encoding FkpA43-Hisb | (11) |
| pGP1–2 | Gene 1 (T7 polymerase) behind λPL controlled by the temperature-sensitive CI857 λ repressor | (53) |
| pPG773 | pT7–7 pbpB-cmi, ApR encoding PbpB′-′Cmi | (33) |
| pSP130/155 | encodes MalE1–26-Cma1–271 with a C-terminal His-tag controlled by the araBAD promoter, araC, Apr | This study |
a Cma with a N-terminal His6 tag.
b FkpA protein with a C-terminal His6 tag.