TABLE 4.
Activity of selected Cma mutant proteins introduced into cells by osmotic shock
The percent of surviving cells of AB2847, its fkpA40 derivative, and the pPG773 cmi transformant were determined by plating after treatment with purified Cma wt and Cma mutant proteins transferred into cells by osmotic shock (+ shock) or without shock treatment (− shock). The average percentage of survivors of all experiments after shock treatment without Cma was 6.0.
| Conditions | Surviving cells |
||
|---|---|---|---|
| AB2847 | AB2847 fkpA | AB2847 pPG773 cmi | |
| Percent of input | |||
| Cma wt + shock | 0 | 1.5 | 6.1 |
| Cma wt − shock | 0.2 | 93.3 | 97.4 |
| Cma(P107A) + shock | 0.03 | 1.8 | 8.8 |
| Cma(P107A) − shock | 1.7 | 91.3 | 93.9 |
| Cma(P129A) + shock | 0.04 | 0.8 | 7.0 |
| Cma(P129A) − shock | 1.9 | 95.2 | 95.8 |
| Cma(P176A) + shock | 0.4 | 0.6 | 8.9 |
| Cma(P176A) − shock | 9.4 | 90.4 | 97.3 |
| Cma(P260A) + shock | 0.03 | 0.2 | 5.8 |
| Cma(P260A) − shock | 1.4 | 90.6 | 100 |