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. 2010 Dec 7;286(8):6650–6658. doi: 10.1074/jbc.M110.191627

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Plasma membrane targeted OS2 sensor reveals localized microdomains of O-GlcNAc activity. Serum-starved Cos7 cells transfected with either PM-OS2 or PM-OS2-control were treated with serum as before and their corresponding FRET changes were quantified (supplemental Movies 5 and 6, respectively). The regions utilized for the analysis of the FRET changes are labeled with arrows. A, upon serum stimulation, increases in FRET were observed within localized regions of the plasma membrane (red arrow). Overall, no change was observed in the plasma membrane of cells overexpressing the PM-OS2 control sensor (white arrow). The scale bar represents 30 μm for both PM-OS2 and PM-OS2 control sensors. The color bar represents the FRET ratio values. B, graphical representation of A. Data shown are representative of a minimum of three cells from independent experiments.