Fig. 3. Rab6-KIFL levels oscillate in a cell cycle controlled manner. (A) HeLa cells were arrested with nocodazole for 20 h, then released into fresh drug-free medium. Samples were collected every hour as shown, and the number of mitotic, interphase and apoptotic cells counted. Cell extracts were analysed by western blotting with antibodies to Rab6-KIFL, cyclin B2 and α-tubulin as a loading control. (B) HeLa cells were arrested with 2 mM hydroxyurea for 18 h, then released into fresh drug-free medium. The cells were analysed as before. Solid arrowheads indicate the time of drug washout, and open arrowheads the peak of mitotic cells.