Fig. 5. Discrete localization of Rab6-KIFL and the Golgi apparatus throughout the cell cycle. (A) HeLa cells fixed with methanol were stained with the affinity-purified sheep antibody to Rab6-KIFL, a rabbit polyclonal antibody to the Golgi marker GM130, while the DNA was stained with DAPI. Representative images of cells at the indicated stages of the cell cycle are shown. (B) HeLa cells fixed with paraformaldehyde were stained with antibodies to Rab6 and α-tubulin; the DNA was stained with DAPI. A representative image of cells at the indicated stage of the cell cycle is shown. Arrows denote areas of particular interest.