TABLE 2.
Plasmids and bacterial strains
| Plasmid | Description | Source or reference |
|---|---|---|
| pBAD24 | AmpRaraC; PBAD promoter | Ref. 51 |
| pBAD24-IISA(Pst) | AmpRaraC; PBAD promoter, spoIISA | This study |
| pET15-H-C-IISA | AmpRlacI; T7 promoter, sequence coding for His6 tag-fused 169-residue C-terminal part of SpoIIA | This study |
| pET15-IISB | AmpRlacI; T7 promoter, spoIISB | This study |
| pET26-C-IISA | KanRlacI; T7 promoter, sequence coding for non-His6-tag-fused 169-residue C-terminal part of SpoIIA | This study |
| pET-Duet-IISABB | AmpRlacI; 2xT7 promoter, sequence coding for His6 tag-fused 169-residue C-terminal part of SpoIIA, two copies of spoIISB | This study |
| pUS19 | AmpR SpcR | Ref. 52 |
| pUS19-FRIISB | AmpR SpcR; flanking regions upstream and downstream of spoIISB | This study |
| pUS19-DIISB | AmpR SpcR; flanking regions upstream and downstream of spoIISB; KanR | This study |
| pUS19-IISA(Pst) | AmpR SpcR; spoIISA | This study |
| pUS19-IISAB22 | AmpR SpcR; spoIISA spoIISB(C22) | This study |
| pUS19-IISAB31 | AmpR SpcR; spoIISA spoIISB(C31) | This study |
| pUS19-IISAB40 | AmpR SpcR; spoIISA spoIISB(N40) | This study |
| pUS19-IISAB44 | AmpR SpcR; spoIISA spoIISB(N44) | This study |
| pUS19-IISAB46 | AmpR SpcR; spoIISA spoIISB(C46) | This study |
| pUS19-IISAB48 | AmpR SpcR; spoIISA spoIISB(N48) | This study |
| pUS19-IISAB52 | AmpR SpcR; spoIISA spoIISB(N52) | This study |
| pUS19-IISAB56 | AmpR SpcR; spoIISA spoIISB(C56) | This study |
| E. coli | ||
| BL21(DE3) | F− ompT hsdS(rB−, mB−) gal dcm (DE3) | Novagen |
| B834(DE3) | F− ompT hsdS(rB−, mB−) gal dcm met (DE3) | Novagen |
| B. subtilis | ||
| PY79 | Prototrophic derivative of B. subtilis 168 | Ref. 53 |
| IB1252 | PY79 spoIISB::kan | This study |