TABLE 1.

Kinetic parameters of bacterial UGLs

	Δ6S			Δ0S
	Km	kcat	kcat/Km	Km	kcat	kcat/Km
	mm	s−1	s−1mm−1	mm	s−1	s−1mm−1
SagUGL
WT	0.100 ± 0.0283	10.2 ± 0.662	102	1.27 ± 0.0941	2.69 ± 0.128	2.12
T235A	1.75 ± 0.344	3.96 ± 0.554	2.26	NDa	ND	ND
S365H	2.36 ± 0.160	3.85 ± 0.199	1.63	0.763 ± 0.215	1.69 ± 0.255	2.21
S368G	0.191 ± 0.0269	24.8 ± 1.12	130	1.40 ± 0.332	10.8 ± 1.71	7.71
K370I	1.47 ± 0.191	41.6 ± 3.84	28.2	0.371 ± 0.0692	3.97 ± 0.297	10.7
BacillusUGL
WT	18.6 ± 6.61	54.9 ± 18.3	2.95	0.381 ± 0.0394	14.1 ± 0.625	36.8
H339S	7.19 ± 1.54	24.5 ± 4.70	3.41	0.861 ± 0.147	16.9 ± 1.40	19.6
G342S	7.00 ± 2.65	20.9 ± 7.09	2.99	0.504 ± 0.0405	18.2 ± 0.665	36.1
I344K	2.20 ± 0.105	39.8 ± 1.41	18.1	0.566 ± 0.0928	23.8 ± 1.86	42.0

^a ND, kinetic parameters could not be determined due to the very low activity.