FIGURE 8.

Requirement of PA binding to the PH domain for the localization of RA-RhoGAP at plasma membrane. A, localization of PH-RA domain and its mutants in COS7 cells. COS7 cells were transfected with HA-PH-RA domain (WT), HA-PH MT-RA domain (PH MT), HA-PH-RA MT domain (RA MT), or HA-PH MT-RA MT domain (PH MT-RA MT) and cultured for 36 h. The cells were immunostained with the anti-HA mAb (green) and phalloidin (red). The staining of F-actin was used for detection of peripheral plasma membrane. Bars, 50 μm. B, percentage of the cells localizing PH-RA domain at peripheral plasma membrane. The analysis of localization was performed on 40–60 transfected cells.