FIGURE 1.

Alignment and spatial location of residues composing the L5 binding pocket of HsEg5 to cognate sequences from Klp61F. A, sequence alignment of the L5 region of HsEg5 to Klp61F is shown. Identical residues are shaded in gray. Residues shaded in yellow mark the L5 loop. Open triangles mark residues within HsEg5 in close contact to STC, and filled black triangles mark residues within HsEg5 in close contact to monastrol. B, shown is an overview of the location of the STC-binding site within HsEg5. STC (magenta) is surrounded by a schematic view of HsEg5 with the L5 loop in yellow and helices α2 and α3 in green. Contact side chains for STC are shown in stick view, with Ala218 of helix α3 in orange. This PyMol-generated image is derived from PDB 3KEN (13).