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22Na+ transport is electrogenic. Membrane vesicles (protein concentration of 2.8 mg/ml) in Pipes/KOH buffer (pH 6.8) containing 25 mm MgSO4, 420 mm sucrose, 1.9 mm NaCl, 150 mm KCl, and 17 μm valinomycin showed 22Na+ transport upon addition of PPi (triangles) and no addition of valinomycin (squares). The arrow indicates the addition of PPi.
