Table 1. Movement patterns of BDC2.5 TH cells and Treg cells in the pancreatic lymph node.
| Recipient mice | Cell type imaged | Total mice analyzed | Swarm and arrest-cluster | Swarm without arrest-cluster | No swarm or arrest-cluster |
|---|---|---|---|---|---|
| NOD | TH | 19 | 1 (5.2%) | 12 (63.2%) | 6 (31.6%) |
| NOD | Treg | 10 | 0 | 6 (60%) | 4 (40%) |
| NOD + BDC2.5 Treg cells | TH | 10 | 0 | 0 | 10 (100%) |
| Cd28−/− | TH | 9 | 9 (100%) | 0 | 0 |
| Cd28−/− | Treg | 5 | 4 (80%) | 1 (20%) | 0 |
| Cd28−/− + NOD Treg cells | TH | 3 | 0 | 3 (100%) | 0 |
| Cd28−/− + BDC2.5 Treg cells | TH | 5 | 0 | 0 | 5 (100%) |
| Cd80−/−Cd86−/− | TH | 3 | 3 (100%) | 0 | 0 |
TPLSM imaging of BDC2.5 CD4+CD25− TH cells and Treg cells in the pancreatic lymph nodes of various recipients. The entire top 350 μm of each lymph node was first surveyed for the presence of cell aggregations indicative of swarming and clustering activity. The movement of the cells in these areas was then recorded by time-lapse imaging to determine the pattern of the movement: free, swarming (swarm), or clustering and arrest (cluster-arrest). The lymph nodes were then classified into three categories based on the type of movement pattern present: swarming plus clustering and arrest; swarming without clustering and arrest; or no swarming or clustering and arrest. The percentage of lymph nodes in each category is in parentheses.