The mutation points of yeast Arf1p, which
cause ts phenotype as a single mutation, and the sequence comparison of
Arf1p from various organisms. Dots indicate identity with S.
cerevisiae Arf1p. Consensus GTP-binding (DVGG, NKQD, and CAT)
and hydrolysis (GX4GK) sequences are marked with asterisks.
The putative effector domain is shown by dashes. The site of Sec7
domain interaction on human ARF1 and the switch 1 and switch 2 GTPase
regions (Mossessova et al., 1998) are marked with double
lines. Sc, S. cerevisiae; Sp, Schizoaccharomyces
pombe; Hs, Homo sapiens; Bt, Bos
taurus; Dm, Drosophila melanogaster; At,
Arabidopsis thaliana; Cr, Chlamydomonas
reinhardtii. The database accession numbers from GenBank are as
follows: Sc Arf1, P11076; Sc Arf2, P19146; Sc Arf3, P40994; Sp Arf1,
AL031534.1; Hs ARF1, NM 001658.1; Bt ARF2, P16500; Hs ARF3,
NM 001659.1; Hs ARF4, NM 001660.1; Hs ARF5, NM 001662.1; Hs ARF6,
NM 001663.1; Dm ARF1, P35676; At ARF1, P36397; Cr ARF1, U27120.1.